We focus on DDI1-like proteins (DNA damage-inducible protein 1) that were largely understudied, but that have been brought up to general interest in the past couple of years. The reason is simple: DDI1-like proteins are newly discovered players involved in DNA repair and also in transcription regulation. The complete mechanism of both DDI1 activities however still remains to be uncovered.
We are also interested in the biology of hepatitis B virus, particularly the covalently closed circular DNA (cccDNA), which resides in the nucleus of infected hepatocytes as a non-integrated plasmid-like molecule and serves as a transcriptional template for HBV. Its elimination is a key step towards possible HBV cure. We thus focus on various strategies for cccDNA degradation, such as development of nanoparticles transporting CRISPR/Cas9 system targeted to cccDNA.